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KMID : 0895820220320040009
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Journal of Oriental Rehabilitation Medicine
2022 Volume.32 No. 4 p.9 ~ p.18
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Anti-inflammatory Effects of Naetakbaekryeom-san
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Jung Min-Jae
Noh Hui-Jeong
Choi Ji-Min
Jeon Seok-Hee
Kim Seon-Jong
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Abstract
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Objectives This study was conducted to confirm the anti-inflammatory effect of Naetakbaekryeom-san (NTB), and whether it could be another treatment for inflammatory diseases.
Methods The NTB water extract was extracted with hot water at 100℃ for 2 hours, concentrated at 80℃ under reduced pressure, and used. After 2 hours of pretreatment with NTB and positive control Bay11-7082, nitric oxide (NO), inducible NO synthase (iNOS), interleukin (IL)-6, IL-1β, tumor necrosis factor alpha (TNF-α) were measured in RAW264.7 cells activated with lipopolysaccharides (LPS) 500 ng/mL. After 2 hours of pretreatment with NTB, the anti-inflammatory effect of NTB was evaluated by measuring nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) in RAW264.7 cells and 293T cells activated with phorbol 12-myristate 13-acetic acid (PMA) 30 ng/mL.
Results In RAW264.7 cells activated with LPS, NTB at concentrations of 0.1, 0.3, and 1.0 mg/mL showed no cytotoxicity, significantly inhibited NO production and inhibition of iNOS expression. TNF-α cytokine levels was not regulated, but NTB at each concentration inhibited the production of IL-1β and IL-6, and the effect was higher than that of the positive control Bay11-7082 (20 μM). In PMA-activated RAW264.7 cells and 293T cells, each concentration of NBT decreased the NF-κB transcriptional activity, with the greatest decrease at 1 mg/mL.
Conclusions These results demonstrated the anti-inflammatory effect of NTB water extracts, but further studies such as comparison of anti-inflammatory effects and antioxidant effects by NTB component, comparison of effects according to extraction solvents, and clinical studies are needed.
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KEYWORD
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Naetakbaekryeom-san, Anti-inflammatory, Herbal medicine
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